Autoantibodies directed against α1-adrenergic receptor and endothelin receptor A in patients with prostate cancer

Autoimmunity Highlights

Table 2 Peptides used to localize (loop analysis, epitope mapping) the binding site of the endothelin receptor A and the α1-adrenergic receptor targeted by ETA-AAB and α1-AAB

A	Endothelin receptor A	α1-adrenergic receptor
Loop 1	¹³⁴LPINVFKLLAGRWPFDHNDFGVFLCKL¹⁶⁰	⁸⁹LGYWAFGRVFCN¹⁰⁰
Loop 2	²²⁹FEYRGEQHKTCMLNATSKFMEFYQDVKD²⁵⁶	¹⁶⁸PAPEDETICQINEE¹⁸¹
Loop 3	³²⁹KKTVYNEMDKNRCELLLSFLL³⁴⁸	²⁹⁸FPDFKPSETVFKIVIFWLGYLNSC³²⁹
B	Endothelin receptor A second loop	α1-adrenergic receptor first loop
Loop	FEYRGEQHKTCMLNATSKFMEFYQDVKD	LGYWAFGRVFCN
P1	FEYRGEQ	FWAFGR
P2	EQHKTCM	GRVFCDV
P3	MLNATSK
P4	SKFMEFY
P5	FYQDVKD

A: Peptides representing the first, second and third extracellular loops of the endothelin receptor A and the α1-adrenergic receptor. B: Peptides with overlapping loop sequence representing the second extracellular loop (endothelin receptor A) and the first extracellular loop (α1-adrenergic receptor)
ETA and α1-adrenergic receptor (ETA receptor: ¹³⁴LPINVFKLLAGRWPFDHNDFGVFLCKL¹⁶⁰, ²²⁹FEYRGEQHKTCMLNATSKFMEFYQDVKD²⁵⁶, and ³²⁹KKTVYNEMDKNRCELLLSFLL³⁴⁸); (α1-adrenergic receptor: ⁸⁹LGYWAFGRVFCN¹⁰⁰, ¹⁶⁸PAPEDETICQINEE¹⁸¹, and ²⁹⁸FPDFKPSETVFKIVIFWLGYLNSC³²⁹)

ISSN: 2038-3274