Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting

Table 1 Comparison of software-generated and visual positive/negative classification

	Visual evaluation (observer 1)			Visual evaluation (observer 2)
	Negative	Positive	Total (N)	Negative	Positive	Total (N)
EUROPattern (EPa) software (adjusted)
Negative	673	2	675	620	55	675
Positive	84	274	358	45	313	358
Total (N)	757	276	1033	665	368	1033
Kappa agreement	0.81			0.79
Relative sensitivity* (%)	99.3			85.1
Relative specificity* (%)	88.9			93.2

*To calculate relative sensitivity and specificity, the visual evaluation made by the observer (1 or 2) is regarded as correct

ISSN: 2038-3274