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Table 1 Final concentrations of primers used in allele specific PCRs

From: HLA-DQ2 and -DQ8 haplotypes frequency and diagnostic utility in celiac disease patients of Gaza strip, Palestine

 

Primer Conc. (μM)

DQA1*05

DQB1*02

DQB1*03:02

AS primer

0.6

0.3

0.45

IC primer

0.3

0.3

0.21

  1. AS allele specific, IC internal control