Table 3 Concordance between different assay platforms for the detection of anti-DFS70/LEDGFp75 autoantibodies in selected ANA-positive human sera
Serum | ANA pattern (1:160 dilution) | DFS70-ELISA (cut-off = OD >15 U/mL, 2 SD) | WB (reactivity with 70–75 kDa band) | DFS70-CIA (cut-off = 20 CU) |
|---|---|---|---|---|
DM-03 | NFS (mc −) | 2.63 | Negative | 1.5 |
DM-037 | Weak NFS (mc −) | 1.60 | Negative | <1.0 |
DM-041 | Weak NFS (mc −) | 3.21 | Negative | <1.0 |
DM-095 | Weak NFS (mc −) | 3.09 | Negative | <1.0 |
DM-104 | Weak NFS (mc −) | 2.41 | Negative | <1.0 |
DM-105 | Weak NFS (mc −) | 3.32 | Negative | 1.5 |
DM-119 | Strong DFS mixed | 80.76 | Strong positive with other multiple reactive bands | 265.3 |
DM-156 | Weak NFS (mc −) | 2.29 | Negative | <1.0 |
RA-860 | NFS (mc −) | 1.80 | Negative | <1.0 |
RA-862 | Strong NFS (mc −) | 1.70 | Negative | 1.2 |
RA-866 | Weak NFS (mc −) | 3.10 | Negative | <1.0 |
RA-869 | Weak NFS (mc −) | 1.30 | Negative | <1.0 |
RA-880 | Weak DFS | 19.18 | Weak positive | 23.0 |
RA-895 | Weak NFS (mc −) | 2.90 | Negative | <1.0 |
RA-903 | Weak NFS (mc −) | 1.90 | Negative | <1.0 |
RA-907 | Very weak nuclear homogeneous | 24.38 | ND | ND |
RA-909 | Strong DFS | 105.79 | Strong positive | 123.8 |
RA-912 | Nuclear coarse speckled | 0.60 | Negative | <1.0 |
OB-033 | Weak NFS (mc −) | 0.92 | Negative | <1.0 |
OB-041 | Strong DFS | 106.87 | Strong positive | 166.2 |
OB-101 | Weak NFS (mc −) | 0.92 | Negative | <1.0 |
OB-156 | Weak NFS (mc −) | 38.72 | Weak positive | 72.8 |
OB-163 | Weak NFS (mc −) | 0.80 | Negative | <1.0 |
OB-165 | Weak homogeneous | 5.84 | Negative | 1.2 |
HI-808 | Weak DFS | 32.27 | Weak positive | 46.3 |
HI-817 | Weak DFS | 32.47 | Moderately strong positive | 45.9 |
HI-820 | NFS (mc −) | 0.50 | Negative | <1.0 |
HI-825 | Weak NFS (mc −) | 1.30 | Negative | <1.0 |
HI-829 | Strong DFS | 103.10 | Strong positive | 308.1 |
HI-840 | Weak DFS | 38.06 | Weak positive | 24.5 |
HI-847 | Weak NFS (mc −) | 1.60 | Negative | <1.0 |
HI-849 | Weak NFS (mc −) | 2.00 | Negative | <1.0 |