Fig. 1

Multiplex reaction environment of the CytoBead CeliAk for the simultaneous analysis of celiac disease (CD)-specific IgA autoantibodies and IgA deficiency. Employing compartmented wells on classical indirect immunofluorescence (IIF) glass slides, tissue transglutaminase type 2 (TG2)- and deamidated Gliadin (DG)-coated fluorescent beads as well as reference beads were immobilized in the left-hand well section. Further, cryopreserved tissue sections of monkey esophagus were fixed in the middle section for classical endomysial antibody (EmA) analysis by IIF as well as antihuman IgA antibody-coated and reference beads in the right-hand well section. Exemplary, the well demonstrates a typical finding of a serum from a patient with CD by showing the classical EmA pattern on monkey esophagus in the middle section and a positive fluorescent halo of TG2-coated beads in the left-hand as well as of anti-IgA beads in the right-hand sections. Reference beads aid in distinguishing TG2-coated beads from DG-coated ones and orientation by visual evaluation with fluorescence microscope