Skin CD30+ T cells and circulating levels of soluble CD30 are increased in patients with graft versus host disease

Table 1 Cytokine secretion profile of T-cell clones derived from skin biopsies and peripheral blood (PB) of patients with acute GvHD

Patients and source of T cells	No. of CD4 clones obtained	Cytokine profile
Patients and source of T cells	No. of CD4 clones obtained	Th1	Th0	Th2
1. Skin	37	2 (5)	25 (68)	10 (27)
PB	41	14 (34)	25 (61)	2 (5)
2. Skin	33	1 (3)	13 (39)	19 (58)
PB	49	18 (37)	27 (55)	4 (8)
3. Skin	42	2 (5)	35 (83)	5 (12)
PB	37	14 (38)	22 (59)	1 (3)
4. Skin	34	0 (–)	28 (82)	6 (18)
PB	52	21 (41)	29 (56)	2 (4)
5. Skin	36	0 (–)	31 (86)	5 (14)
PB	41	16 (39)	22 (54)	3 (7)
6. Skin	44	1 (2)	22 (50)	21 (48)
PB	36	12 (33)	21 (58)	3 (8)
All cases
Skin	226	6 (3)	154	66 (29)
PB	256	95 (37)	146	15 (6)
χ²		86.02	6.30	46.78
P		<0.0001	<0.02	<0.0001

T-cell blasts (10⁶/ml) of each clone were stimulated for 36 h with PMA (10 ng/ml) plus anti-CD3 MoAb (200 ng/ml) and culture supernatants were assayed for IFN-γ, IL-4, and IL-5 content. T-cell clones able to produce IFN-γ, but not IL-4 or IL-5, were categorized as Th1; clones able to produce IL-4 and/or IL-5, but not IFN-γ, were categorized as Th2, and clones producing both IFN-γ and IL-4 or IL-5 were categorized as Th0
Percent values in parenthesis

ISSN: 2038-3274