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Table 3 Immunoprecipitation assay and related molecular weights with lysosomes/endosomes autoantibodies and LC3B marker antibody from HEp-2 cell lysate

From: Characterization for anti-cytoplasmic antibodies specificity by morphological and molecular techniques

Protein (kDa)

Sample 1

Sample 2

Sample 3

Sample 4

LC3B marker

Catepsin-L

  

~31

 

~24–33

Catepsin-L

Trace 42

Trace 42

Trace 42

Trace 42

 

LC3B

65

65

65

65

65

LC3B

    

80

LAMP-2

Trace 105

Trace 105

Trace 105

Trace 105

 

GRASP-1

Trace 110

Trace 110

Trace 110

Trace 110