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Fig. 4 | Autoimmunity Highlights

Fig. 4

From: Endogenous mitochondrial double‐stranded RNA is not an activator of the type I interferon response in human pancreatic beta cells

Fig. 4

PNPT1/SUV3 knockdown induces dsRNA accumulation in surrounding fibroblasts but not in dispersed human islet cells. Dispersed human islets were transfected with a siRNA control (siQ) or with siRNAs targeting PNPT1 (#1), SUV3 or both (P/S), and then maintained in culture for 6 days after transfection. dsRNA accumulation (red), insulin content for beta cell staining (green), and vimentin for fibroblast staining (purple) were analyzed by immunocytochemistry. Representative images of 4 (a) and 2 (b, c) independent experiments are shown (magnification 40×). c dsRNA and vimentin positivity were evaluated by counting 1400 non-beta cells from 69 images obtained from 2 independent experiments

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Autoimmunity Highlights

ISSN: 2038-3274

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