Name | Apparent molecular weight (kD) in immunoblots | Cellular functions in the context of interaction with DFS70/LEDGF | Interacting domain | References |
---|---|---|---|---|
CDC7/ASK | 65–70 | Subunit and activator of S-phase kinase ASK involved in DNA replication, repair, and recombination; phosphorylates DFS70/LEDGF which in turn stimulates the kinase enzymatic activity | IBD | |
CtIP | 120–130 | Endonuclease that interacts with DFS70/LEDGF to facilitate repair of DNA double strand breaks through homologous recombination | PWWP | [55] |
JPO2/CDCA7L | 52 | Transcription factor that binds to c-Myc, mediates its transforming effect in medulloblastoma cells, and brings it in close proximity to DFS70/LEDGF in transcription complexes | IBD | |
IWS1 | 92 | Chromatin remodeler and component of RNA pol II complex; interacting partner of Spt6 transcription elongator that modulates histone methylation and production of mature mRNA transcripts; associated with Spt6 and DFS70/LEDGF for post-integration silencing of HIV-1 gene expression in HIV latency | IBD | |
Menin | 68–70 | Positively regulates Hox gene expression; required for MLL-fusion protein mediated leukemic transformation through ternary complex with MLL1 and DFS70/LEDGF | IBD | [75] |
MLL | > 400 | Plays key role in early development, hematopoiesis, and leukemogenesis; involved in transcriptional activation of Hox genes and cancer-associated genes through complex with Menin and DFS70/LEDGF | IBD | |
MeCP2 | 55–70 | Methylation-associated transcriptional modulator that interacts with DFS70/LEDGF to influence its transcriptional activity | PWWP | [30] |
PC4 | 75 | Transcription co-activator component of RNA pol II complex that interacts with both DFS70/LEDGF and its p52 splice variant during general transcription | Likely PWWP | [7] |
PogZ | 155 | Transposase that regulates chromatin remodeling and is required for proper chromosome segregation during mitosis; its DDE endonuclease domain, necessary for efficient DNA transposition, binds DFS70/LEDGF | IBD | |
TOX4 | 65–75 | Transcription factor involved in transcription activation and DNA repair in complex with DFS70/LEDGF | PWWP | [56] |
NOVA1 | 50–55 | Alternative mRNA splicing cofactor that interacts with DFS70/LEDGFp75 to link RNA pol II transcription with Mrna processing | PWWP | [56] |
RNA pol II | 250 | Polymerase that catalyzes the transcription of DNA to mRNA | Likely PWWP | |
SRSF1 and multiple splicing factors | Vary | SRSF1 is one of a plethora of mRNA splicing factors that bind to both DFS70/LEDGF and its splice variant p52, suggesting involvement of PWWP domain; DDX5 and SNRPA splicing factors bind only to DFS70/LEDGF, suggesting involvement of IBD | Likely both PWWP or IBD | [57] |
SSRP1 | 94 | Structure specific recognition protein 1 component of the chromatin remodeler FACT (facilitates transcription complex) | PWWP | [156] |