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Table 2 Known interacting partners of DFS70/LEDGF

From: Twenty years of research on the DFS70/LEDGF autoantibody-autoantigen system: many lessons learned but still many questions

Name

Apparent molecular weight (kD) in immunoblots

Cellular functions in the context of interaction with DFS70/LEDGF

Interacting domain

References

CDC7/ASK

65–70

Subunit and activator of S-phase kinase ASK involved in DNA replication, repair, and recombination; phosphorylates DFS70/LEDGF which in turn stimulates the kinase enzymatic activity

IBD

[45, 61]

CtIP

120–130

Endonuclease that interacts with DFS70/LEDGF to facilitate repair of DNA double strand breaks through homologous recombination

PWWP

[55]

JPO2/CDCA7L

52

Transcription factor that binds to c-Myc, mediates its transforming effect in medulloblastoma cells, and brings it in close proximity to DFS70/LEDGF in transcription complexes

IBD

[45, 46, 61, 88]

IWS1

92

Chromatin remodeler and component of RNA pol II complex; interacting partner of Spt6 transcription elongator that modulates histone methylation and production of mature mRNA transcripts; associated with Spt6 and DFS70/LEDGF for post-integration silencing of HIV-1 gene expression in HIV latency

IBD

[45, 46, 61]

Menin

68–70

Positively regulates Hox gene expression; required for MLL-fusion protein mediated leukemic transformation through ternary complex with MLL1 and DFS70/LEDGF

IBD

[75]

MLL

> 400

Plays key role in early development, hematopoiesis, and leukemogenesis; involved in transcriptional activation of Hox genes and cancer-associated genes through complex with Menin and DFS70/LEDGF

IBD

[45, 46, 61, 75]

MeCP2

55–70

Methylation-associated transcriptional modulator that interacts with DFS70/LEDGF to influence its transcriptional activity

PWWP

[30]

PC4

75

Transcription co-activator component of RNA pol II complex that interacts with both DFS70/LEDGF and its p52 splice variant during general transcription

Likely PWWP

[7]

PogZ

155

Transposase that regulates chromatin remodeling and is required for proper chromosome segregation during mitosis; its DDE endonuclease domain, necessary for efficient DNA transposition, binds DFS70/LEDGF

IBD

[45, 46]

TOX4

65–75

Transcription factor involved in transcription activation and DNA repair in complex with DFS70/LEDGF

PWWP

[56]

NOVA1

50–55

Alternative mRNA splicing cofactor that interacts with DFS70/LEDGFp75 to link RNA pol II transcription with Mrna processing

PWWP

[56]

RNA pol II

250

Polymerase that catalyzes the transcription of DNA to mRNA

Likely PWWP

[7, 86]

SRSF1 and multiple splicing factors

Vary

SRSF1 is one of a plethora of mRNA splicing factors that bind to both DFS70/LEDGF and its splice variant p52, suggesting involvement of PWWP domain; DDX5 and SNRPA splicing factors bind only to DFS70/LEDGF, suggesting involvement of IBD

Likely both PWWP or IBD

[57]

SSRP1

94

Structure specific recognition protein 1 component of the chromatin remodeler FACT (facilitates transcription complex)

PWWP

[156]