Fig. 4

Detection of DFS70/LEDGF interacting partners using protein arrays. Commercially available transcription factor arrays were used in early studies to identify candidate interacting transcription factors of DFS70/LEDGF. Purified histidine tagged-DFS70/LEDGF was incubated with transcription factors spotted on membranes. Protein interactions were then detected with human anti-DFS70/LEDGF autoantibody, horseradish peroxidase-labeled anti-human secondary antibody, followed by exposure to chemiluminescence reagents. A representative image of a transcription factor array membrane showing positive interactions with components of the RNA pol II complex (TFII) as well as c-Myc and PC4. This approach was used for the initial detection of the interaction of DFS70/LEDGF with MeCP2 [30]