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Table 2 Characteristics of survey participants

From: The antinuclear antibody HEp-2 indirect immunofluorescence assay: a survey of laboratory performance, pattern recognition and interpretation

Characteristics

CL, N (%)a

IVD, N (%)a

HEp-2 kit

Bio-Rad

4 (25.00)

See legend

Euroimmun

5 (31.25)

Inova

6 (37.50)

MBL Bion

1 (6.25)

Type of reader

Manual only

9 (56.25)

5 (62.50)

Manual and Automated

7 (43.75)

3 (37.25)

Cut-off (titer)

< 1:10

1 (6.25)

0 (00.00)

< 1:40

12 (75.00)

6 (66.67)

< 1:80

3 (18.75)

2 (22.22)

< 1:100

0 (00.00)

1 (11.11)

Technologist experienceb

1–5 years

7 (33.30)

0 (00.00)

6–10 years

4 (12.10)

1 (11.11)

> 10 years

10 (47.60)

8 (88.89)

Median (range), years

10 (1-45)

20 (2-51)

Patterns reported

Nuclear only

3 (18.75)

0 (00.00)

cCytoplasmic and nuclear

2 (12.50)

0 (00.00)

Mitotic and nuclear

3 (18.75)

0 (00.00)

cAll patterns

8 (50.00)

8 (100.00)

  1. aNumber (N) of clinical laboratories (CL) or in vitro diagnostic manufacturers (IVD) unless otherwise stated. Participating IVD manufacturers included: AESKU Diagnostics, Bio-Rad, Euroimmun, Inova, ImmunoConcepts, Scimedx, ThermoFisher and Zeus. CL labs using Inova HEp-2 substrate kits use < 1:40 or < 1:80 as cut-off for ANA determinations. bMore than one technologist was involved in the reading and interpretation of the results in some laboratories. cOne laboratory in each group reports cytoplasmic pattern only as a comment
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