The antinuclear antibody HEp-2 indirect immunofluorescence assay: a survey of laboratory performance, pattern recognition and interpretation

Table 1 Survey specimens and their characteristics

Specimen^a	Cellular staining	Traditional nomenclature	ICAP nomenclature	ICAP level
ANA-001	Mitotic	Mitotic	Spindle fiber, AC-25	Expert
ANA-002	Nuclear	Discrete nuclear dots	Multiple nuclear dots, AC-6	Expert
ANA-003	Nuclear	Speckled	Coarse speckled, AC-5	Expert
ANA-004	Cytoplasmic	Cytoplasmic	Reticular/AMA, AC-21	Competent
ANA-005	Nuclear	Centromere	Centromere, AC-3	Competent
ANA-006	Nuclear	Nucleolar	Homogeneous nucleolar, AC-8	Expert
ANA-007	Nuclear	Speckled	DFS, AC-2	Competent
ANA-008	Cytoplasmic	Cytoplasmic	DFS, AC-19	Expert
ANA-009	Cytoplasmic	Cytoplasmic	Fine speckled, AC-20	Expert
ANA-010	Nuclear	Speckled	Fine speckled, AC-4	Expert
ANA-011	Nuclear	Speckled/Other*	Anti-topoisomerase I, AC-29^b	Expert
ANA-012	Mitotic	Mitotic	NuMA-like, AC-26^c	Expert

^anumber, ICAP International Consensus on Antinuclear Antibody Pattern, AC anti-cell, AMA anti-mitochondrial antibodies, DFS dense fine speckled, NuMA nuclear mitotic apparatus protein. ^bThe AC-29 Anti-topoisomerase pattern I is a compound pattern, classified within ICAP as a speckled pattern. The complex pattern involves speckled nuclear staining, and also includes staining of the condensed chromatin, cytoplasmic staining, staining of the nucleolar organizing region in mitotic cells, and variable nucleolar staining of interphase cells. ^cFor this specimen, AC-25 was also considered acceptable

ISSN: 2038-3274