Performance analysis of automated evaluation of antinuclear antibody indirect immunofluorescent tests in a routine setting

Table 3 Comparison of software-generated and visual pattern identification

	Visual evaluation (observer 1)
	H	S	N	C	D	NM	Cyt
	n = 157	n = 63	n = 37	n = 16	n = 5	n = 2	n = 59
EUROPattern (adjusted) (EPa) software
TP	147	55	34	15	4	2	51
FP	80	90	20	1	4	3	11
TN	796	880	976	1016	1024	1028	963
FN	10	8	3	1	1	0	8
Relative sensitivity (%)	93.6	87.3	91.9	93.8	80.0	100.0	86.4
Relative specificity (%)	90.9	90.7	98.0	99.9	99.6	99.7	98.9
Kappa agreement	0.71	0.49	0.74	0.94	0.61	0.57	0.83

H homogeneous, S speckled, N nucleolar, C centromere, D nuclear dots, NM nuclear membrane, Cyt cytoplasmic, TP true positive, FP false positive, TN true negative, FN false negative, observer 1 is considered the reference method

ISSN: 2038-3274