Table 1 IIF ANA patterns and relevant clinical associations
From: Personalized medicine in rheumatology: the paradigm of serum autoantibodies
Pattern | Related antigens | Related diagnosis |
|---|---|---|
Nuclear | ||
 Homogeneous | dsDNA, histones, nucleosomes | SLE, drug-induced lupus, JIA |
 Speckled | hnRNP, U1RNP, Sm, SS-A, SS-B, RNAP-III, Mi-2, Ku | MCTD, SLE, SjS, DM, SSc/PM overlap |
  Dense fine speckled | DFS70/LEDGF | Rare in SLE, SjS, SSc |
  Fine speckled | SS-A, SS-B, Mi-2, TIF1γ, TIF1β, Ku, RNA helicase A, replication protein A | SjS, SLE, DM, SSc/PM overlap |
  Large/coarse speckled | hnRNP, U1RNP, Sm, RNAP-III | MCTD, SLE, SSc |
 Centromere | CENP-A/B | lcSSc, PBC |
 Discrete nuclear dots | ||
  Multiple nuclear dots | Sp100, PML protein, MJ/NXP-2 | PBC, SARD, PM/DM |
  Few nuclear dots | p80-coilin, SMN | SjS, SLE, SSc, PM, asymptomatic subjects |
 Nucleolar | ||
  Homogeneous | PM/Scl75, PM/Scl100, Th/To, B23 nucleophosmin, nucleolin, No55/SC65 | SSc, SSc/PM overlap |
  Clumpy | U3-snoRNP/fibrillarin | SSc |
  Punctate | RNAP-I, hUBF/NOR-90 | SSc, SjS |
 Nuclear envelope (NE) | ||
  Smooth NE | Lamins A, B, C, or lamin associated proteins | SLE, SjS, seronegative arthritis |
  Punctate NE | Nuclear pore complex proteins | PBC |
 Pleomorphic | ||
  PCNA-like | PCNA | SLE, other conditions |
  CENP F-like | CENP-F | Cancer, other conditions |
Cytoplasmic | ||
 Fibrillar | ||
  Linear/actin | Actin, non-muscle myosin | MCTD, CH, cirrhosis, MG, CD, PBC, long-term HD |
  Filamentous/microtubules | Vimentin, cytokeratin | Infections or inflammations, long-term HD, ALD, SARD, PsO, healthy subjects |
  Segmental | α actinin, vinculin, tropomyosin | MG, CD, UC |
 Speckled | ||
  Discrete dots | GW182, Su/Ago2, Ge-1 | PBC, SARD, neurological and autoimmune conditions |
  Dense fine speckled | PL-7, PL-12, ribosomal P proteins | ASS, PM/DM, SLE, juvenile SLE, neuroSLE |
  Fine speckled | Jo1/histidyl-tRNA synthetase | ASS, PM/DM, lcSSc, IPE |
 Reticular/AMA | PDC-E2/M2, BCOADC-E2, OGDC-E2, E1α subunit of PDC, E3BP/proteinX | PBC, SSc, rare in other SARD |
 Polar/Golgi-like | Giantin/macrogolgin, golgin-95/GM130, golgin-160, golgin-97, golgin-245 | Rare in SjS, SLE, RA, MCTD, GPA, ICA, PCD, viral infections |
 Rods and rings | IMPDH2, others | HCV patients post IFN/ribavirin, rare in SLE, Hashimoto’s and healthy controls |
Mitotic | ||
 Centrosome | Pericentrin, ninein, Cep250, Cep110, enolase | Rare in SSc, RD, infections (viral and mycoplasma) |
 Spindle fibers | HsEg5 | Rare in SjS, SLE, other SARD |
  NuMA-like | Centrophilin | SjS, SLE, other |
 Intercellular bridge | Aurora kinase B, CENP-E, MSA2, KIF14, MKLP1 | Rare in SSc, RD, malignancies |
 Mitotic chromosomal envelope | Modified histone H3, MCA1 | Rare in DLE, CLL, SjS, PMR |