Fig. 7

HEp-2 ELITE/DFS70 KO substrate as the first step IIF screening method simplifies the ANA screening and confirmation algorithm. Ability to detect and confirm DFS70 mono-specific reactions while simultaneously screening for disease associated ANAs reduces the number of confirmatory assays. Grey boxes represent a large subset of ANA screening population who may not need a follow up in absence of a strong clinical suspicion for AARD/SARD. Using the new substrate, these cases do not need additional testing. Classic ANA positive cases with or without DFS70 pattern will be confirmed by appropriate solid phase confirmatory assays