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Fig. 6 | Autoimmunity Highlights

Fig. 6

From: Analysis of DFS70 pattern and impact on ANA screening using a novel HEp-2 ELITE/DFS70 knockout substrate

Fig. 6

Shows examples of homogeneous, mitochondrial, centromere, speckled, nucleolar and DFS70 (mono-specific) reactions on the new HEp-2 ELITE/DFS70 KO substrates. Arrows represent conventional HEp-2 cell nuclei intensely stained with DFS70 reactive serum. For classic ANA patterns both conventional and engineered HEp-2 cells show identical reactions. Bottom panel shows examples of mixed patterns revealed on the HEp-2 ELITE DFS70 KO substrates when DFS70 pattern co-exist with another classic ANA pattern. Arrows indicate conventional HEp-2 with strong DFS70 pattern. Less intensely labeled DFS70 KO cells are able to reveal fine speckled, nucleolar and nuclear envelope/homogeneous reactions

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