Fig. 5

A schematic represents the design of the novel HEp-2 ELITE/DFS70 KO substrate. a–d Schematics for common patterns (DFS70, homogeneous and speckled) on both interphase and mitotic HEp-2 nuclei in conventional and DFS70 KO cells is described. e Example of a DFS70 mono-specific reaction on conventional HEp-2 substrate. f Example of a DFS70 mono-specific reaction on HEp-2 ELITE/DFS70 KO substrate is shown. Arrows indicate conventional HEp-2 cell nuclei. Negatively stained nuclei are derived from the engineered DFS70 KO cells that do not express LEDGF/psip1/p75 antigen