Fig. 5
From: The immunofluorescence techniques in the diagnosis of endocrine autoimmune diseases
Double IF on human unfixed pancreas. a Green pattern produced by a serum of a patient counterstained by FITC-labeled goat anti-human serum, the fluorescence is concentrated in the cytoplasm of scattered cells of the islet. b Red pattern produced by an anti-somatostatin rabbit serum counterstained with a rhodamine-labelled anti-rabbit serum the rabbit serum stains in red the same cells as the human serum. c The double microphotographic exposure of the images a and b, produced a yellow colour (sum of green and red) confirming that the human serum reacts against somatostatin-producing cells