Fig. 4
From: The immunofluorescence techniques in the diagnosis of endocrine autoimmune diseases
Double IF on human unfixed pancreas. a Green pattern produced by a serum of a patient counterstained by FITC-labeled goat anti-human serum, the fluorescence is concentrated on the cytoplasm of scattered cells of the islet. bRed pattern produced by an anti-glucagon rabbit serum counterstained with a rhodamine-labeled anti-rabbit serum: the rabbit serum stains in red the same cells as the human serum. c The double microphotographic exposure of the images a and b, produces a yellow colour (sum of the green and the yellow) confirming that the human serum reacts against glucagon-producing cells