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Table 1 TSHR residues important for biological activity of 5C9, MAb-B2, K1-70 and M22

From: Blocking type TSH receptor antibodies

TSHR residues important for blocking activity of 5C9 (mutation experiments) TSHR residues important for blocking activity of MAb-B2 (mutation experiments) TSHR residues that interact with K1-70 (from the K1-70-TSHR crystal structure and mutation experiments) TSHR residues that interact with M22 (from the M22-TSHR crystal structure and mutation experiments)
  D36 D36 D36
  R38 R38 R38
   K42  
   Q55  
    T56
  K58 K58 a K58
   I60a  
   E61  
  R80 R80 R80 a
  Y82 Y82 a Y82 a
   S84  
   T104  
   H105 H105 a
   E107 E107 a
  R109 R109 a R109 a
   N110 N110
K129 K129 K129 K129 a
   F130 F130 a
  F134   
   D151 D151 a
    I152
F153   F153 F153 a
   I155 I155
   E157 E157
E178    
   T181  
K183   K183 a K183 a
    Y185a
D203   D203  
    N208
    K209a
    Q235
E251    E251
    R255a
    N256
  1. The residues shown in bold are important for more than one antibody. Interactions between TSHR residues and K1-70 or M22 residues in their respective crystal structures include hydrogen bonds, salt bridges, non-hydrogen bonding polar interactions, hydrophobic contacts, van der Waals interactions (showing variation of accessible surface area on complexation >60 Å2) and charge–charge interactions (strength > 6.0e−10 N by residues or >2.5e−10 N by atoms)
  2. aAmino acids, which when mutated, affected the biological activity of K1-70 or M22