Blocking type TSH receptor antibodies

Autoimmunity Highlights

Table 1 TSHR residues important for biological activity of 5C9, MAb-B2, K1-70 and M22

TSHR residues important for blocking activity of 5C9 (mutation experiments)	TSHR residues important for blocking activity of MAb-B2 (mutation experiments)	TSHR residues that interact with K1-70 (from the K1-70-TSHR crystal structure and mutation experiments)	TSHR residues that interact with M22 (from the M22-TSHR crystal structure and mutation experiments)
	D36	D36	D36
	R38	R38	R38
		K42
		Q55
			T56
	K58	K58 ^a	K58
		I60^a
		E61
	R80	R80	R80 ^a
	Y82	Y82 ^a	Y82 ^a
		S84
		T104
		H105	H105 ^a
		E107	E107 ^a
	R109	R109 ^a	R109 ^a
		N110	N110
K129	K129	K129	K129 ^a
		F130	F130 ^a
	F134
		D151	D151 ^a
			I152
F153		F153	F153 ^a
		I155	I155
		E157	E157
E178
		T181
K183		K183 ^a	K183 ^a
			Y185^a
D203		D203
			N208
			K209^a
			Q235
E251			E251
			R255^a
			N256

The residues shown in bold are important for more than one antibody. Interactions between TSHR residues and K1-70 or M22 residues in their respective crystal structures include hydrogen bonds, salt bridges, non-hydrogen bonding polar interactions, hydrophobic contacts, van der Waals interactions (showing variation of accessible surface area on complexation >60 Å²) and charge–charge interactions (strength > 6.0e−10 N by residues or >2.5e−10 N by atoms)
^aAmino acids, which when mutated, affected the biological activity of K1-70 or M22

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