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Fig. 3 | Autoimmunity Highlights

Fig. 3

From: Myositis-specific autoantibodies: detection and clinical associations

Fig. 3

Schematic representation of a addressable-bead autoantigen immunoassay. Purified autoantigens are coupled to differentially labelled microbeads that can be detected by illumination with a laser. Patient sera can be incubated with mixtures of beads each coated with a different autoantigen. One laser is used to identify the specific antigen coupled to each bead based upon the fluorescent properties of the bead, and a second laser is used to determine the binding of autoantibodies to the beads after incubation with secondary antibodies conjugated to a distinct fluorophore

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