Fig. 3From: Myositis-specific autoantibodies: detection and clinical associationsSchematic representation of a addressable-bead autoantigen immunoassay. Purified autoantigens are coupled to differentially labelled microbeads that can be detected by illumination with a laser. Patient sera can be incubated with mixtures of beads each coated with a different autoantigen. One laser is used to identify the specific antigen coupled to each bead based upon the fluorescent properties of the bead, and a second laser is used to determine the binding of autoantibodies to the beads after incubation with secondary antibodies conjugated to a distinct fluorophoreBack to article page